Not to a huge degree, admittedly, but enough that more than a few of us have ended up with a more in-depth knowledge of assembly than one might expect.
This has typically arisen from working with existing OSS projects which incorporate vector transforms and the ilk in ASM, often badly or subtly brokenly, and from building optimised functions for doing fun things like creating n-dimensional maps of customer/product relationships in order to determine purchase propensity based on cluster profiles.
So yeah, ASM in a web shop. Except we're only kinda a web shop these days - client sites are our marketing platform, but our business is business systems.