ideally, a laminar flow cabinet, but reasonably careful technique and the pen/strep would probably see you through
So, when? Depends if you have Prime really.
The thing is, growing sheets of cells is not a big step towards doing anything interesting. You need to do chemical and genetic manipulation of the cells, and that's the hard part.
fresh fertilized chicken eggs
lab gloves
an egg incubator
a dissecting microscope
tissue culture dishes
phosphate-buffered saline
a pipette pump
10 ml pipettes
two pairs of dissecting tweezers
70% ethanol solution
kimwipes
lab gloves
a steady hand
tissue culture medium (DMEM is fine)
fetal calf serum
penicillin-streptomycin mix
a micropipette
micropipette tips
a tissue culture incubator
a tank of carbon dioxide
ideally, a laminar flow cabinet, but reasonably careful technique and the pen/strep would probably see you through
So, when? Depends if you have Prime really.
The thing is, growing sheets of cells is not a big step towards doing anything interesting. You need to do chemical and genetic manipulation of the cells, and that's the hard part.